When -Glu-Asp- appears at the N-terminal of the sequence, attention should be paid to avoid too alkaline environment and purity reduction during purification. The first two amino acids of the N-terminal of Amyloid β-Protein (22-35) sequence are Glu and Asp. In acid system,Amyloid β-Protein (22-35) will be relatively stable.
Amyloid β-Protein (22-35) is a hydrophobic segment of β - amyloid protein, which is a difficult point in the study. A reasonable dissolution ratio is an important part of the study.In the synthesis,Fmoc-Ile-Ile-OH can be used, and β-Amyloid (22-35) in acid system will be more stable when preserved.