Based on the sequence of β-Amyloid Protein, a lot of changes have been made to the Amyloid Bri Protein,The addition of Cys and the formation of disulfide bond make the research effect of Amyloid Bri Protein different.
There are three glutamic acids in the Amyloid Bri Protein (1-23) sequence, which are easy to form glutamine and lead to the decrease of purity. In the process of production and preservation, the influence of condensation agent, solution system and storage conditions on the stability of peptide should be considered.
As for the formation of disulfide bond, the air oxidation method can be used for the formation of Amyloid Bri Protein (1-23). The formation of disulfide bond by air oxidation is the most classical method in peptide synthesis, and has achieved good results in the early research. The method of air oxidation is usually used to dissolve the peptide in the reduced state in water and react for more than 24 hours under the condition of near neutral or weak alkaline (pH 6.5-10). In order to reduce the possibility of disulfide bond formation between molecules, it usually needs to be carried out at low concentration.